Culturing neuronal cells on surfaces coated by a novel polyethyleneimine-based polymer.

نویسندگان

  • Y Bledi
  • A J Domb
  • M Linial
چکیده

Maintaining cells in culture is essential for studying many aspects of cell biology and physiology. Cell culturing is dependent on proper anchorage of cells to the growth surfaces. For most cell lines, and especially for post-mitotic neurons, coated tissue culture plates are prerequisite for seeding. The most commonly used coating reagents are positively charged polymers such as poly-L-lysine or biologically purified adhesive molecules such as collagen. In this report, we present a simple procedure for synthesizing and for coating cell culture surfaces. The reagent is a biologically inert hydrophobized polyethyleneimine (PEI), which provides adequate adhesive properties for cultured cell lines including those of neuronal lineage. The hydrophobized PEI is branched PEI modified by octadecanyl groups bound to 2 mol% of the amino groups of the PEI. Unlike the native PEI that is water soluble, the modified PEI is soluble in ethanol, and thus resistant to solubilization in biological media. The protocol of coating was optimized for tissue culture plates as well as glass surfaces and in many respects this polymer outperformed other routinely used coating reagents. Neuronal cell lines, plated on the polymer-treated surfaces are resistant to manipulations including repeated media changes and extensive washing. The advantage of coating surfaces with the developed PEI-based polymer compared to other commonly used coating reagents is discussed.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Adhesion and proliferation of cells on new polymers modified biomaterials.

Up to today, several techniques have been used to maintain cells in culture for studying many aspects of cell biology and physiology. More often, cell culture is dependent on proper anchorage of cells to the growth surface. Poly-l-lysine is commonly used as adhesive molecule. In this study, we present, as an alternative to poly-l-lysine, new polymer film substrates, realized by electropolymeriz...

متن کامل

Culture of neural cells on polymers coated surfaces for biosensor applications.

We focused our study on the olfactory cells growth on biocompatible polymer films electrodeposited on a silicon microsystem. Several substrates such as polyethyleneimine (PEI), polypropyleneimine (PPI), and polypyrrole (PPy), acting as potentially good candidates for cell culture, were tested in order to allow cells to adhere and proliferate. During their growth, the evolution of their morpholo...

متن کامل

Regulation of angiogenesis through the efficient delivery of microRNAs into endothelial cells using polyamine-coated carbon nanotubes

MicroRNAs (miRNAs) directly regulate gene expression at a post-transcriptional level and represent an attractive therapeutic target for a wide range of diseases. Here, we report a novel strategy for delivering miRNAs to endothelial cells (ECs) to regulate angiogenesis, using polymer functionalized carbon nanotubes (CNTs). CNTs were coated with two different polymers, polyethyleneimine (PEI) or ...

متن کامل

مقایسه‌ی کشت سلول‌های استرومایی مغز استخوان با نانوالیاف PLGA پوشش داده شده با ژلاتین و پلی ال لایزین

Background and Objective: Poly D,L-lactic-co-glycolic acid (PLGA) is known as biodegradable and biocompatible polymer. These polymers have recently received much attention in tissue engineering. The aim of this study was to investigate the biological behavior of the bone marrow stromal cells (BMSCs) in culturing with PLGA nanofibers coated with poly-L-lysine or gelatin. Materials and Methods: I...

متن کامل

Nanofiltration Membranes Synthesized from Polyethyleneimine for Removal of MgSO4 from Aqueous Solution (RESEARCH NOTE)

A novel work was performed for fabrication and modification of composite nanofiltration (NF) membrane by polymerization reaction between polyethyleneimine (PEI) and triphthaloyldechloride (TPC). the main purpose of this work was water treatment. polysulfone was applied as a main polymer of substrate. The result of reaction between PEI and TPC would be formation of polyamide layer on the membran...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Brain research. Brain research protocols

دوره 5 3  شماره 

صفحات  -

تاریخ انتشار 2000